Interaction of the family-B DNA polymerase from the archaeon Pyrococcus furiosus with deaminated bases
S Gill, R O'Neill, RJ Lewis, BA Connolly - Journal of molecular biology, 2007 - Elsevier
The interaction of archaeal family B DNA polymerases with deaminated bases has been
examined. As determined previously by our group, the polymerase binds tightly to uracil (the …
examined. As determined previously by our group, the polymerase binds tightly to uracil (the …
[9] DNA polymerases from hyperthermophiles
HH Hogrefe, J Cline, AE Lovejoy, KB Nielson - Methods in enzymology, 2001 - Elsevier
Publisher Summary With the emergence of the polymerase chain reaction (PCR),
considerable attention has been focused on isolating and characterizing the DNA …
considerable attention has been focused on isolating and characterizing the DNA …
Two Family B DNA Polymerases from Aeropyrum pernix, an Aerobic Hyperthermophilic Crenarchaeote
IKO Cann, S Ishino, N Nomura, Y Sako… - Journal of …, 1999 - Am Soc Microbiol
ABSTRACT DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a
hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and …
hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and …
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
G Henneke, D Flament, U Hübscher, J Querellou… - Journal of molecular …, 2005 - Elsevier
DNA polymerases carry out DNA synthesis during DNA replication, DNA recombination and
DNA repair. During the past five years, the number of DNA polymerases in both eukarya and …
DNA repair. During the past five years, the number of DNA polymerases in both eukarya and …
Uracil recognition in archaeal DNA polymerases captured by X-ray crystallography
SJ Firbank, J Wardle, P Heslop, RJ Lewis… - Journal of molecular …, 2008 - Elsevier
Archaeal family B DNA polymerases bind tightly to template-strand uracil and stall
replication on encountering the pro-mutagenic base. This article describes an X-ray crystal …
replication on encountering the pro-mutagenic base. This article describes an X-ray crystal …
Nucleotide sequence of the gene for a 74 kDa DNA polymerase from the archaeon Sulfolobus solfataricus.
D Prangishvili, HP Klenk - Nucleic acids research, 1993 - ncbi.nlm.nih.gov
Recently, the nucleotide sequence of a DNA polymerase gene from Sulfolobus solfatanicus
was reported (1). The enzyme is a 100 kDa polypeptide also possessing a 3'-5'exonuclease …
was reported (1). The enzyme is a 100 kDa polypeptide also possessing a 3'-5'exonuclease …
Family A and family B DNA polymerases are structurally related: evolutionary implications
W Zhu, J Ito - Nucleic acids research, 1994 - academic.oup.com
In order to establish the evolutionary relationship between the family A and B DNA
polymerases, we have closely compared the 3'→ 5'exonuclease domains between the …
polymerases, we have closely compared the 3'→ 5'exonuclease domains between the …
The DNA polymerase gene from the hyperthermophilic marine archaebacterium, Pyrococcus furiosus, shows sequence homology with alpha-like DNA polymerases.
EJ Mathur, MW Adams, WN Callen… - Nucleic acids …, 1991 - ncbi.nlm.nih.gov
Recent studies in the evolutionary relationships of life on this planet have placed the root of
the universal tree between eubacteria (or Bacteria) on the one hand and archaebacteria (or …
the universal tree between eubacteria (or Bacteria) on the one hand and archaebacteria (or …
Structural basis for uracil recognition by archaeal family B DNA polymerases
MJ Fogg, LH Pearl, BA Connolly - Nature structural biology, 2002 - nature.com
Deamination of cytosine to uracil in a GC base pair is a major promutagenic event,
generating GC→ AT mutations if not repaired before DNA replication. Archaeal family B …
generating GC→ AT mutations if not repaired before DNA replication. Archaeal family B …
A heterodimeric DNA polymerase: evidence that members of Euryarchaeota possess a distinct DNA polymerase
IKO Cann, K Komori, H Toh… - Proceedings of the …, 1998 - National Acad Sciences
We describe here a DNA polymerase family highly conserved in Euryarchaeota, a
subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2 …
subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2 …