The interaction of archaeal family B DNA polymerases with deaminated bases has been
examined. As determined previously by our group, the polymerase binds tightly to uracil (the …

Publisher Summary With the emergence of the polymerase chain reaction (PCR),
considerable attention has been focused on isolating and characterizing the DNA …

ABSTRACT DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a
hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and …

DNA polymerases carry out DNA synthesis during DNA replication, DNA recombination and
DNA repair. During the past five years, the number of DNA polymerases in both eukarya and …

Archaeal family B DNA polymerases bind tightly to template-strand uracil and stall
replication on encountering the pro-mutagenic base. This article describes an X-ray crystal …

Recently, the nucleotide sequence of a DNA polymerase gene from Sulfolobus solfatanicus
was reported (1). The enzyme is a 100 kDa polypeptide also possessing a 3'-5'exonuclease …

In order to establish the evolutionary relationship between the family A and B DNA
polymerases, we have closely compared the 3'→ 5'exonuclease domains between the …

Recent studies in the evolutionary relationships of life on this planet have placed the root of
the universal tree between eubacteria (or Bacteria) on the one hand and archaebacteria (or …

Deamination of cytosine to uracil in a GC base pair is a major promutagenic event,
generating GC→ AT mutations if not repaired before DNA replication. Archaeal family B …

We describe here a DNA polymerase family highly conserved in Euryarchaeota, a
subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2 …