Extracts with a relatively large content of DNA polymerase were prepared by incubating
purified nuclei from calf thymus at low ionic strength and 2° C for several hours and raising …

The novel DNA polymerase recently isolated from the nuclei of calf thymocytes in our
laboratory was characterized and its distinctive properties were determined. The enzyme …

Purified calf thymus DNA Polymerase a is inactive with native DNA as template and shows
little activity with denatured DNA. DNA synthesis with denatured DNA as template is greatly …

A method is described for the preparation of DNA polymerase purified about 800‐fold from
rat liver nuclei. The yield of enzyme is about 140–200 μg from 200 g liver. Sodium …

Modification of the purification procedures for rabbit bone marrow DNA polymerase [Byrnes,
JJ, & Black, VL (1978) Biochemistry 17, 4226–4231] has increased the yield and stability of …

1. DNA polymerase from nuclear and supernatant fractions of cultured mouse L929 cells
was fractionated on columns of Sephadex G-200, Sepharose 4B and of DEAE-cellulose …

Cytoplasmic DNA polymerase preparation from calf thymus was separated into three peaks
on DEAE-cellulose column. All these three species preferred activated DNA to denatured …

Two forms of DNA polymerase have been partially purified about 100-to 200-fold from calf
thymus by fractionation with streptomycin, acid precipitation, and chromatography on …

A replicative DNA polymerase has been partially purified from the nonhistone chromosomal
proteins of rat liver. The enzyme exhibited an absolute requirement for DNA and divalent …

Marietta YW T. Lee, Cheng-Keat Tan, Kathleen M. Downey, and Antero G. So* abstract: DNA
polymerase 5 from calf thymus has been purified to apparent homogeneity by a new …