We have studied the effect of DNA methylation on human y-globin gene expression, using a
novel in vitro DNA methylation technique. With this method we have methylated specific …

When the human beta-globin gene was methylated at every cytosine residue and was
inserted into mouse fibroblasts by DNA-mediated gene transfer, the transcription of the gene …

The relationship between DNA methylation and differential expression of rabbit beta-like
globin genes was studied by using restriction enzymes that cleave the sequence CCGG but …

The above experiments support a relatively simple model to explain the role of DNA
methylation in vivo. Most tissue-specific genes are methylated. The methyl groups may …

Summary Eukaryotic genomes contain 5-methylcytosine (5mC) as a rare base. 5mC arises
by postsynthetic modification of cytosine and occurs, at least in animals, predominantly in …

The regulation of gene expression in eukaryotes is carried out by a large number of
independent mechanisms operating at different levels. One of the important factors which …

The occurrence of 5-methylcytosine in DNA was first described in 1948 by Hotchkiss (see
first chapter). Recognition of its possible physiologi cal role in eucaryotes was first …

DNA methylation is found almost ubiquitously in nature and the methyltransferases show
evidence of a common evolutionary origin. It will be a fascinating study in protein evolution …

Human gamma A‐globin genes with different patterns of methylation were synthesized in
vitro. These methylated constructs were transfected into mouse L cells and their expression …

Publisher Summary The chapter discusses on the DNA methylation and its possible
biological roles. The pattern of DNA methylation is species-specific. In the DNA of some …