BACKGROUND Human pluripotent stem cells (hPSC), both embryonic and induced (hESC
and hiPSC), are regarded as a valuable in vitro model for early human development. In …

Human pluripotent stem cells (hPSCs) regularly and irreversibly show the erosion of X
chromosome inactivation (XCI) by long non-coding RNA (lncRNA) XIST silencing, causing …

This unit describes a method of performing fluorescent in situ hybridization (FISH) of XIST
and Cot‐1 RNA in human pluripotent stem cells (hPSC) to characterize the epigenetic status …

X chromosome inactivation (XCI) is an essential mechanism for dosage compensation of X-
linked genes in female cells. We report that subcultures from lines of female human …

Dosage compensation of sex-chromosome gene expression between male and female
mammals is achieved via X chromosome inactivation (XCI) by employing epigenetic …

Human pluripotent stem cells (hPSCs) display extensive epigenetic instability, particularly
on the X chromosome. In this study, we show that, in hPSCs, the inactive X chromosome has …

X-chromosome inactivation is an epigenetic hallmark of mammalian development.
Chromosome-wide regulation of the X-chromosome is essential in embryonic and germ cell …

Inactivation of one X chromosome in female mammals (XX) compensates for the reduced
dosage of X-linked gene expression in males (XY). However, the inner cell mass (ICM) of …

Although the reactivation of silenced X chromosomes has been observed as part of the
process of reprogramming female somatic cells into induced pluripotent stem cells (iPSCs) …

STUDY HYPOTHESIS Does a preferential X chromosome inactivation (XCI) pattern exist in
female human pluripotent stem cells (hPSCs) and does the pattern change during long-term …