To mimic post-ovulatory ageing, we have extended the in vitro maturation (IVM) phase to 48
h and examined effects on (i) developmental potential,(ii) expression of a panel of …

Epigenetic changes are critical for the acquisition of developmental potential by oocytes and
embryos, yet these changes may be sensitive to maternal ageing. Here, we investigated the …

The production of bovine embryos through in vitro maturation and fertilization is an important
tool of the genomic revolution in dairy cattle. Gene expression analysis of these embryos …

Assisted reproductive technologies are associated with an increased incidence of
epigenetic aberrations, specifically in imprinted genes. Here, we used the bovine oocyte as …

During the transit through the oviduct, the early embryo initiates an extensive DNA
methylation reprogramming of its genome. Given that these epigenetic modifications are …

Oocyte quality is one of the most important aspects of in vitro embryo development.
Extensive epigenetic programming must occur during oocyte growth and maturation. A …

DNA methylation reprogramming occurs during mammalian gametogenesis and
embryogenesis. Sex-specific DNA methylation patterns at specific CpG islands controlling …

Epigenetic changes, such as DNA methylation, play an essential role in the acquisition of full
developmental competence by mammalian oocytes during the late follicular growth phase …

To explore the mechanisms leading to the poor quality of IVF blastocysts, the single-cell
whole-genome methylation sequencing technique was used in this study to analyse the …

Background Aberrant DNA methylation patterns of genes required for development are
common in in vitro produced embryos. In this regard, we previously identified altered DNA …