Dual-mode FEN1 activity detection based on Nt. BstNBI-induced tandem signal amplification
H Yang, C Wang, E Xu, W Wei, Y Liu, S Liu - Analytical Chemistry, 2021 - ACS Publications
Flap endonuclease 1 (FEN1) is a structure-specific nuclease that cleaves the 5′ single-
stranded protrusion (also known as 5′ flap) during Okazaki fragment processing. It is …
stranded protrusion (also known as 5′ flap) during Okazaki fragment processing. It is …
Signal-amplified detection of the tumor biomarker FEN1 based on cleavage-induced ligation of a dumbbell DNA probe and rolling circle amplification
Flap endonuclease 1 (FEN1), an endogenous nuclease with the ability to cleave the 5′
overhang of branched dsDNA, is of significance in DNA replication and repair. The …
overhang of branched dsDNA, is of significance in DNA replication and repair. The …
Detection of cancer marker flap endonuclease 1 using one-pot transcription-powered clustered regularly interspaced short palindromic repeat/cas12a signal …
S Ding, Y Wei, G Chen, F Du, X Cui, X Huang… - Analytical …, 2022 - ACS Publications
As a critical functional protein in DNA replication and genome stability, flap endonuclease 1
(FEN1) has been considered a promising biomarker and druggable target for multiple …
(FEN1) has been considered a promising biomarker and druggable target for multiple …
Dual signal amplification-integrated single-molecule biosensing of flap endonuclease 1 in breast cancer tissues
F Ma, W Liu, H Liu, Q Xu, C Zhang - Sensors and Actuators B: Chemical, 2023 - Elsevier
Abstract Flap endonuclease 1 (FEN1) is a structure-specific nuclease that may act as a
valuable biomarker for cancer diagnosis. Herein, we construct a dual signal amplification …
valuable biomarker for cancer diagnosis. Herein, we construct a dual signal amplification …
Label-free and homogeneous detection of flap endonuclease 1 by ligation-promoted hyperbranched rolling circle amplification platform
X Wei, Y Meng, Q Xu, J Hu, C Zhang - Talanta, 2022 - Elsevier
The structure-specific endonuclease FEN1 participates in various genome maintenance
pathways in eukaryotes and is associated with different human diseases. Herein, we …
pathways in eukaryotes and is associated with different human diseases. Herein, we …
An ultrasensitive biosensor with suppressed background signals for FEN1 detection in a homogeneous reaction via cascade primer exchange reaction and CRISPR …
YL Song, XL He, MM Pan, M Wang, M Jiang… - Sensors and Actuators B …, 2024 - Elsevier
Abstract Flap endonuclease 1 (FEN1) is a structure-specific enzyme which plays a crucial
role in DNA replication and repair within mammalian cells. FEN1 is also known to be …
role in DNA replication and repair within mammalian cells. FEN1 is also known to be …
A magnetic separation-assisted cascade hybridization chain reaction amplification strategy for sensitive detection of flap endonuclease 1
L Zhang, X Liu, N Zhang, X Liu, W Jiang - Sensors and Actuators B …, 2022 - Elsevier
Sensitive and accurate detection of flap endonuclease 1 (FEN1) is essential to understand
its roles in DNA replication and repair and explore its functions in tumor diagnosis and …
its roles in DNA replication and repair and explore its functions in tumor diagnosis and …
A DNA nano firework for imaging and inhibitor screening of flap endonuclease 1 in living cells
C Wang, D Hao, Y Bao, X Xu, Y Li, B Zou… - Analytical …, 2023 - ACS Publications
In situ observation of changes in the activity of marker proteins in living cells is crucial for
both biomarker-based disease diagnosis and drug screening. Flap endonuclease 1 (FEN1) …
both biomarker-based disease diagnosis and drug screening. Flap endonuclease 1 (FEN1) …
Multimodal detection of flap endonuclease 1 activity through CRISPR/Cas12a trans-cleavage of single-strand DNA oligonucleotides
Abstract Flap endonuclease 1 (FEN1) is an endonuclease that specially removes 5′ single-
stranded overhang of branched duplex DNA (5′ flap). While FEN1 is essential in various …
stranded overhang of branched duplex DNA (5′ flap). While FEN1 is essential in various …
Fluorometric detection of cancer marker FEN1 based on double-flapped dumbbell DNA nanoprobe functionalized with silver nanoclusters
Abstract Flap endonuclease 1 (FEN1), a ubiquitous enzyme involved in DNA repair and
replication, is overexpressed in highly proliferative cancer cells. FEN1 has been recognized …
replication, is overexpressed in highly proliferative cancer cells. FEN1 has been recognized …