Simple screening method for differentially methylated regions of the genome using a small number of cells

T Hamada, S Murasawa, T Asahara - Biochemical and biophysical …, 2007 - Elsevier
Genomic DNA methylation is a major epigenetic mechanism controlling the expression of
genetic information. Therefore, identifying regions of the genome that are differentially …

High-throughput method for detecting DNA methylation

P Hou, M Ji, S Li, N He, Z Lu - Journal of biochemical and biophysical …, 2004 - Elsevier
Aberrant DNA methylation of CpG site is among the earliest and most frequent alterations in
cancer. Detection of promoter hypermethylation of cancer-related gene may be useful for …

Issues in testing DNA methylation using next-generation sequencing

D Baumann, RW Doerge - 2011 - newprairiepress.org
DNA methylation is an epigenetic modification known to affect gene expression, cellular
differentiation, as well as phenotypes. Recent advancements in next-generation sequencing …

A high-throughput DNA methylation analysis of a single cell

M Kantlehner, R Kirchner, P Hartmann… - Nucleic acids …, 2011 - academic.oup.com
In recent years, the field of epigenetics has grown dramatically and has become one of the
most dynamic and fast-growing branches of molecular biology. The amount of diseases …

Introduction to single-cell DNA methylation profiling methods

J Ahn, S Heo, J Lee, D Bang - Biomolecules, 2021 - mdpi.com
DNA methylation is an epigenetic mechanism that is related to mammalian cellular
differentiation, gene expression regulation, and disease. In several studies, DNA …

Variations in DNA methylation patterns during the cell cycle of HeLa cells

SE Brown, MF Fraga, ICG Weaver, M Berdasco… - Epigenetics, 2007 - Taylor & Francis
DNA methylation has been viewed as a stable component of the epigenome, which is
established during development and fixed thereafter. We show here using nearest neighbor …

Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression

F Song, JF Smith, MT Kimura… - Proceedings of the …, 2005 - National Acad Sciences
Early studies proposed that DNA methylation could have a role in regulating gene
expression during development [Riggs, AD (1975) Cytogenet. Cell Genet. 14, 9–25] …

[HTML][HTML] Analysis of tissue-specific differentially methylated regions (TDMs) in humans

E Kitamura, J Igarashi, A Morohashi, N Hida, T Oinuma… - Genomics, 2007 - Elsevier
Alterations in DNA methylation have been implicated in mammalian development. Hence,
the identification of tissue-specific differentially methylated regions (TDMs) is indispensable …

High-throughput assay of DNA methylation based on methylation-specific primer and SAGE

X Wang, C Zhang, L Zhang, X Wang, S Xu - Biochemical and biophysical …, 2006 - Elsevier
Mapping of genomic DNA methylation is a dispensable part of functional genome. We have
developed a novel method based on methylation-specific primer and serial analysis of gene …

Analysis of DNA methylation in mammalian cells

PM Lizardi, Q Yan… - Cold Spring Harbor …, 2017 - cshprotocols.cshlp.org
Methylation of DNA, the most experimentally accessible epigenetic alteration of eukaryotic
cells, has generated an extensive literature and an abundance of analytical tools. The term …