When normal diploid fibroblasts from mice, hamsters, and humans were grown in culture,
the 5-methylcytosine content of their DNA's markedly decreased. The greatest rate of loss of …

Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been
used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine …

Certain cytosine residues of eukaryotic DNA are methylated in inactive regions of the
genome. For a long time the fruitfly Drosophila was thought to be an exception,,,, but now the …

Neoplastic cells simultaneously harbor widespread genomic hypomethylation, more
regional areas of hypermethylation, and increased DNA-methyltransferase (DNA-MTase) …

THE rare bases which occur in animal DNA deserve special attention because their
presence is directly relevant to questions concerning the cellular, subcellular and species …

A correlation has been shown between changes in the methylation pattern of cytosine
residues in DNA and the expression of specific genes in differentiated tissues1–6. The …

Hypermethylation of tumor suppressor genes and other genes functionally important in the
neoplastic process is a recently recognized process. This epigenetic process is …

Heritable alterations of DNA that do not affect the base pair sequence itself but nevertheless
regulate the predetermined activity of genes are referred to as epigenetic. Epigenetic …

The levels of DNA methyltransferase in nuclei from 9 tumorigenic and 9 nontumorigenic cell
lines were examined. In all but 2 cases, the extractable methyltransferase activity was 4 …

The degree of methylation of active regions of the chromosome has been investigated by
several techniques. DNase I (deoxyribonuclease I, EC 3.1. 21.1) was used to introduce nicks …