The analysis of completed archaeal genome sequences led to the identification of a set of
approximately 10–20 genes whose protein products were inferred to be involved in …

Thermus aquaticus DNA polymerase was shown to contain an associated 5′ to 3′
exonuclease activity. Both polymerase and exonuclease activities cosedimented with a …

Polymerases from the Pol I family which are able to effi ciently use ddNTPs have
demonstrated a much improved performance when used to Sequence DNA. A number of …

DNA polymerase of the archaeon Thermococcus litoralis can synthesize a long stretch of
linear double-stranded DNA in the complete absence of added primer and template DNAs …

It is now well recognized that the information processing machineries of archaea are far
more closely related to those of eukaryotes than to those of their prokaryotic cousins, the …

Phylogenetic analysis of Y-family DNA polymerases suggests that it can be subdivided into
several discrete branches consisting of UmuC/DinB/Rev1/Rad30/Rad30A and Rad30B. The …

The DNA sliding clamp is a multifunctional protein involved in cellular DNA transactions. In
Archaea and Eukaryota, proliferating cell nuclear antigen (PCNA) is the sliding clamp. The …

A widespread and highly conserved family of apparently inactivated derivatives of archaeal
B-family DNA polymerases is described. Phylogenetic analysis shows that the inactivated …

DNA replication is essential for all life forms. Although the process is fundamentally
conserved in the three domains of life, bioinformatic, biochemical, structural, and genetic …

Although archaeal genomes encode proteins similar to eukaryotic replication factors, the
hyperthermophilic archaeon Pyrococcus abyssi replicates its circular chromosome at a high …