Insertional mutagenesis is a method for identifying genes essential for a given biological
process by using the integration of DNA as the mutagen, thereby facilitating the cloning of …

Two recent large-scale genetic screens in zebrafish have identified many mutations that
affect differentiation in a variety of organ systems, particularly the notochord, the neural crest …

The zebrafish is an ideal organism for small molecule studies. The ability to use the whole
organism allows complex in vivo phenotypes to be assayed and combines animal testing …

The zebrafish, Danio rerio, has become a major model organism used in biomedical studies.
The widespread use of Danio rerio in research laboratories requires a comprehensive …

Because of its many favorable characteristics, the zebrafish has become a popular model for
studies of vertebrate development (1, 2). To further enhance the utility of the zebrafish model …

Phenotype‐driven chemical genetic screens in zebrafish have become a proven approach
for both dissection of developmental mechanisms and discovery of potential therapeutics. A …

Although mammals have been cloned from genetically manipulated cultured cells, a
comparable achievement has not been realized in lower vertebrates. Here we report that …

Recently our laboratory described an efficient method for generating retroviral provirus
insertions in the zebrafish germ line, and we showed that provirus insertions induce …

Despite the pre-eminence of the mouse in modelling human disease, several aspects of
murine biology limit its routine use in large-scale genetic and therapeutic screening. Many …

Culturing cells in vitro can produce a uniform population for the study of cellular
differentiation, which is especially useful for the quantification of gene expression or the …