Thermus aquaticus DNA polymerase was shown to contain an associated 5′ to 3′
exonuclease activity. Both polymerase and exonuclease activities cosedimented with a …

The 5'----3'exonuclease activity of the thermostable enzyme Thermus aquaticus DNA
polymerase may be employed in a polymerase chain reaction product detection system to …

A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7. 7.7) with a temperature optimum
of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The …

The two high-molecular-weight DNA polymerases from Euglena gracilis, pol A (mol. wt. 190
000) and pol B (mol. wt. 240 000), were differentiated on the basis of associated enzymic …

The Micrococcus luteus DNA polymerase possesses a 5′ to 3′ exonuclease activity which
is an integral part of the polymerase protein. The 5′ to 3′ exonuclease preferentially …

The Thermus aquaticus DNA polymerase I (Taq Pol I) gene was cloned into a plasmid
expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated …

The DNase that is associated with a multiprotein form of HeLa cell DNA polymerase alpha
(polymerase alpha 2) has two distinct exonuclease activities: the major activity initiates …

A thermophilic DNA polymerase has been purified to near homogeneity from the
archaebacterium Thermoplasma acidophilum. Analysis of the purified enzyme by sodium …

Materials and Methods Materials. Taq DNA polymerase (lots 2A and 3) was purchased from
New England Biolabs. E. coli DNA polym-erase I, large (Klenow) fragment (Kf), was …

Xenopus laevis DNA polymerase γ co-purifies with a tightly associated 3′→ 5′
exonuclease. The purified enzyme lacks 5′→ 3′ exonuclease and endonuclease activity …