DNA polymerase∊(pol∊) from HeLa cells was purified to near homogeneity, utilizing Mono
S fast protein liquid chromatography for complete separation from pol α. The purified pol∊ …

The cDNA of human DNA polymerase δ was cloned. The cDNA had a length of 3.5 kb and
encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 …

The contributions of human DNA polymerases (pols) α, δ and ε during S‐phase progression
were studied in order to elaborate how these enzymes co‐ordinate their functions during …

The major pol α activity of CHO cells was purified 2 800-fold to near homogeneity and was
characterized with respect to its physical and catalytic properties. The purified enzyme, upon …

During the past decade, five DNA polymerases (pol) have been characterized in eukaryotic
cells. These enzymes are called a, p, y, 6, and E (Wang 1991). Their enzymatic properties …

The catalytic polypeptide of human DNA polymerase δ was overexpressed in BSC-40 cells
(African green monkey kidney cell line) using the vaccinia virus/pTM1 system. The …

MATERIALS AND METHODS Materials. Reagents were analytical grade I.[32P] dTTP and
[3H] dTTP were from Amersham. FIAU was provided by Oclassen Pharmaceuticals, San …

The POL1 gene of the fission yeast, Schizosaccharomyces pombe, was isolated using a
POL1 gene probe from the budding yeast Saccharomyces cerevisiae, cloned and …

A 12-kDa and two 25-kDa polypeptides were isolated with highly purified calf thymus DNA
polymerase δ by conventional chromatography. A 16-mer peptide sequence was obtained …

Publisher Summary This chapter discusses the purification of mammalian polymerases,
DNA polymerase δ. DNA polymerase δ is one of three DNA polymerases required for …