Methylation of cytosines is the key epigenetic modification of DNA in eukaryotes and is
associated with a repressed chromatin state and inhibition of gene expression. The …

Cytosine residues in the sequence 5′ CpG (cytosine–guanine) are often postsynthetically
methylated in animal genomes. CpG methylation is involved in long-term silencing of certain …

MeCP2 is an abundant mammalian protein that binds to methylated CpG. We have found
that native and recombinant MeCP2 repress transcription in vitro from methylated promoters …

Background Transgenes are often engineered using regulatory elements from distantly
related genomes. Although correct expression patterns are frequently achieved even in …

Although it is known that the methylation of DNA in 5′ promoters suppresses gene
expression, the role of DNA methylation in gene bodies is unclear,,,,. In mammals, tissue …

The 5'region of the gene encoding human X chromosome-linked phosphoglycerate kinase 1
(PGK1) is a promoter-containing CpG island known to be methylated at 119 of 121 CpG …

DNA methylation is the epigenetic modification, which introduces 5mC as fifth base onto
DNA. As for the distribution of 5mCs, it is well known that they distribute themselves in a non …

CpG islands are normally methylation free in cells of the animal, even when the associated
gene is transcriptionally silent. In mouse NIH 3T3 and L cells, however, over half of the …

Methylation of mammalian DNA can lead to repression of transcription and alteration of
chromatin structure. Recent evidence suggests that both effects are the result of an …

Silencing of tumor-suppressor genes by hypermethylation of promoter CpG islands is well
documented in human cancer and may be mediated by methyl-CpG-binding proteins, like …