MATERIALS AND METHODS Chemicals-[Me-3H] Thymidine-5• Œ-triphos phate([3H] dTTP)
and other labeled compounds were purchased from New England Nuclear Co., USA …

Two forms of DNA polymerase have been partially purified about 100-to 200-fold from calf
thymus by fractionation with streptomycin, acid precipitation, and chromatography on …

Revised Manuscript Received August 1, 1986 abstract; Purified calf thymus DNA
polymerases I and II each have an associated 3'to 5'exonuclease but otherwise resemble …

We have purified a DNA polymerase α species from calf thymus to near homogeneity. The
enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in …

A novel DNA polymerase, which could use both poly (rA)· oligo (dT) and activated calf
thymus DNA efficiently as template-primers, was purified 20 000-fold from calf thymus …

Methods Polymerization Assay. For thekinetic studies, reaction mixtures were incubated for
5 or 10 min at 37 C and con-tained, in a final volume of 10 pL, the following: 50 mM Tris-HCl …

The heterogeneity of calf thymus DNA polymerase‐α has been further investigated. In
particular, an enzyme (enzyme D) which exhibits higher activity on poly (dA)·(dT) 10 (A: T …

DNA polymerase alpha, delta and epsilon can be isolated simultaneously from calf thymus.
DNA polymerase delta was purified to apparent homogeneity by a four-column procedure …

Extracts with a relatively large content of DNA polymerase were prepared by incubating
purified nuclei from calf thymus at low ionic strength and 2° C for several hours and raising …

DNA polymerase from BHK-21/C13 cells were separated into two species, DNA polymerase
I corresponding to the heterogeneous enzyme with sedimentation coefficient of 6-8S, and …