Methylation of DNA is a biochemical modification that can influence gene expression and is
involved in inactivating one of the two X chromosomes in women. Evidence that has …

Abstract The DNA base 5-hydroxymethylcytosine (5hmC) is produced by enzymatic
oxidation of 5-methylcytosine (5mC) by 5mC oxidases (the Tet proteins). Since 5hmC is …

We have purified GST-fused recombinant mouse Dnmt3a and three isoforms of mouse
Dnmt3b to near homogeneity. Dnmt3b3, an isoform of Dnmt3b, did not have DNA …

Background: Alterations in global DNA methylation are implicated in various pathobiological
processes. We describe a liquid chromatography-electrospray ionization-tandem mass …

Here we present MethylQuant, a novel method that allows accurate quantification of the
methylation level of a specific cytosine within a complex genome. This method relies on the …

The biological significance of 5-methylcytosine was in doubt for many years, but is no
longer. Through targeted mutagenesis in mice it has been learnt that every protein shown by …

The spatial arrangement and three-dimensional structure of DNA in the nucleus is controlled
through the interdigitation of DNA binding proteins such as histones and their modifiers, the …

Acute myeloid leukemia (AML) is a heterogeneous disease that is characterized by distinct
cytogenetic or genetic abnormalities. Recent discoveries in cancer epigenetics …

Chemical modification of nucleotide bases in DNA provides one mechanism for conveying
information in addition to the genetic code. 5-methylcytosine (5mC) represents the most …

Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been
used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine …