DNA methylation in plants: mechanisms and tools for targeted manipulation

J Gallego‐Bartolomé - New Phytologist, 2020 - Wiley Online Library
… tools for locus-specific manipulation of DNA methylation. These can also … DNA methylation
in plants, focusing on recent advances in the development of tools for its targeted manipulation

Gene-specific targeting of DNA methylation in the mammalian genome

A Urbano, J Smith, RJ Weeks, A Chatterjee - Cancers, 2019 - mdpi.com
DNA methylation and transcription has been difficult thus far. Fortunately, the recent development
of targeted tools for manipulating DNA methylation … regulation of DNA methylation in …

Technologies for targeting DNA methylation modifications: basic mechanism and potential application in cancer

J Wang, J Yang, D Li, J Li - Biochimica et Biophysica Acta (BBA)-Reviews …, 2021 - Elsevier
manipulating gene epigenetic alterations in a more specific and efficient method. In this review,
we described different targeting DNA methylation … understanding of these targeting tools, …

DNA methylation and de-methylation using hybrid site-targeting proteins

Y Lei, YH Huang, MA Goodell - Genome biology, 2018 - Springer
DNA methylation and transcription. Here, we focus on recent advances in targeted DNA
methylation … they have provided into how forcible methylation and demethylation govern gene …

Harnessing targeted DNA methylation and demethylation using dCas9

C Pflueger, T Swain, R Lister - Essays in Biochemistry, 2019 - portlandpress.com
… , implementation, and targeting specificity. Effective targeted DNA methylation editing will
continue … strategies, considerations, and future directions for targeted DNA methylation editing. …

Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein

Y Lei, X Zhang, J Su, M Jeong, MC Gundry… - Nature …, 2017 - nature.com
… of a systematic understanding of DNA methylation and the dynamic changes of … efficient
targeted DNA methylation by fusing inactive Cas9 (dCas9) with an engineered prokaryotic DNA

Reshaping the Landscape of the Genome: Toolkits for Precise DNA Methylation Manipulation and Beyond

C Zhu, Z Hao, D Liu - JACS Au, 2023 - ACS Publications
… and demethylation processes, an intuitive way to enable targeted manipulation of DNA
methylation is by fusing DNBPs with DNMTs or TETs. In such a fusion protein system, the …

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

A Vojta, P Dobrinić, V Tadić, L Bočkor… - Nucleic acids …, 2016 - academic.oup.com
methylation of a larger part of the promoter. DNA methylation activity was specific for the
targeted … that directed DNA methylation of a wider promoter region of the target loci IL6ST and …

CRISPR-dCas9-based targeted manipulation of DNA methylation in plants

B Ghoshal, J Gardiner - CRISPR-Cas Methods: Volume 2, 2021 - Springer
… protocol for targeted manipulation of DNA methylation by … lab for the targeted manipulation
of DNA methylation in Arabidopsis… further study into the function of DNA methylation in plants. …

Large-scale manipulation of promoter DNA methylation reveals context-specific transcriptional responses and stability

A de Mendoza, TV Nguyen, E Ford, D Poppe… - Genome Biology, 2022 - Springer
… These artificial epigenome modification techniques allow targeted interrogation of
whether DNA methylation correlates with, or is causative for, transcriptional repression. Their …