… vitro kinase assay, we have identified serines (Ser) 158, 221 and 318 as targets of protein
kinase C (PKC… HuR constructs bearing a phosphomimetic Ser (S)-to-Asp (D) substitution at the …

… efforts to dissect the different states of the kinase-substrate … catalytic states of the PKC-substrate
interaction. Specifically, … Hence, a phosphomimetic version of peptide number 14 was …

… Using synthetic protein kinase C (PKC) inhibitors and small interfering RNA approaches, we
… This provides evidence that PKCα among the four PKC isoenzymes abundantly expressed …

… by different signalling pathways provides routes to functional … identified after activation of
PKC (protein kinase C) with bryostatin … If this Ser 221 is mutated to a phosphomimetic aspartate …

… ), and anti-PKCδ immunoprecipitate (PKC) were analyzed by Western blotting probing with
… These data indicate that PKCδ affects HuR mRNA binding, allowing the association of HuR …

… in the presence of a phosphomimetic Y197 residue. By contrast, … ) and serine/threonine
kinases (eg protein kinase C, (PKC)), … the requirement for dissecting integrin heterodimer-ligand …

… In summary, 3′-UTRs seem to be major players in gene regulation that enable local
functions, compartmentalization, and cooperativity, which makes them important tools for the …

… by a unique domain specific to each Src-family kinase (SFK) … the myristoylated peptide by
protein kinase C (PKC) at both … , we prepared phosphomimetic CaM mutants by substituting …

… In dissecting further phosphoregulatory cell signals for CAP1… the phosphomimetic and the
nonphosphorylatable mutant of … ), an activator of protein kinase C that we previously found to …

… is consistent with its accessibility to Protein Kinase C δ (PKCδ) to become phosphorylated. …
-to-Asp single mutation. The assignment of the NMR spectrum of S318D allowed us to build a …