… (GPCR)based genetically encoded fluorescent indicators (… components, engineering
strategies, and applications. We … on GPCR- based genetically encoded fluorescent indicators (…

… future, new optimization approaches for GPCR-based biosensors could be considered, such
as structure-guided protein engineering… green fluorescent genetically encoded biosensor …

… successful, perspective, and hopeful attempts at using genetically … genetically encoded
fluorescent assays used for the search of modulators of ion channels, Ca 2+ homeostasis, GPCR …

… the discovery of fluorescent proteins, engineering of their … genetically encoded biosensor
concepts, including those for intensiometric and ratiometric sensors based on single fluorescent …

… for genetically encoded indicators, providing the foundation for the engineering and application
… We assume both PBP-based sensors and GPCR-based sensors have a Hill coefficient of …

… Due to the wide range of ligands that they bind and respond to, G-protein coupled receptors
(GPCR) have long been recognized as an intriguing binding domain for construction of …

… Finally, we give our perspective on where future work is … compartments, whereas GPCR-based
biosensors are membrane … where we believe advances in biosensor engineering are …

… Neurotransmitters and neuromodulators mostly interact with GPCRs of the A group (GPCR-A…
develop the first genetically encoded acetylcholine biosensor. A FRET-based acetylcholine …

… biosensors construction and discuss their application in … and prospects for the use of
genetically encoded fluorescent … the cell genome via gene engineering. The process under study …

… different classes of genetically encoded fluorescent indicators of brain chemistry from two
perspectives: (1) … Protein engineers have employed GPCRs as the sensing domain in multiple …