… Having shown that import of both barley PORA and … of Pchlide of the pea plastids and studied
the import efficiency. Chloroplasts normally contain low or undetectable levels of Pchlide in …

… Here we demonstrate that the precursor of PORA from barley is imported into isolated …
barley chloroplasts is independent of Pchlide. The study of protein import into isolated organelles …

… two putative receptor proteins during the import of PORA and … leaves the import of this protein
no longer requires Pchlide. In … into plastids of both organs occurs independently of Pchlide. …

… NADPH:protochlorophyllide oxidoreductase is a key enzyme for the light-induced greening
of etiolated angiosperm plants. In barley, two POR proteins exist termed PORA and PORB …

… PORA and PORB proteins accumulate and form PLBs as a ternary complex with Pchlide and
NADPH in … For analysis of porC-1 and porC-2, total RNA was isolated from two independent …

… encoded NADPH:protochlorophyllide (Pchlide) oxidoreductase A plastid precursor (pPORA)
can use two different plastid import … Note the lack of imported and processed PORA in mock-…

… is NADPH:protochlorophyllide (Pchlide) oxidoreductase (POR… residue in the PORA could
provide a Pchlide binding site, we … Each POR protein bound two Pchlide molecules per enzyme …

… with Pchlide and NADPH in the light before import, the ATP-dependent binding of the precursor
protein … -reducing activities and two distinct NADPH-protochlorophyllide oxidoreductase …

… protein interacting with pPORA during its Pchlide-dependent … showing tight protein:protein
interactions between CDF1 and PORA, … We provided evidence that this Pchlide-independent …

… restored the Pchlide-dependent import of pPORA. As a result, PORA–Pchlide b–NADPH
complexes … We used transA–DHFR to demonstrate substrate-independent import of this model …