… assemble at discrete sites on the membrane known as ER exit sites (ERES). Here, we
identify Sec16 as the protein that defines ERES in mammalian cells. Sec16 localizes to ERES …

… Protein export from the endoplasmic reticulum (ER), the first step in protein transport through
the secretory … As COPII assembly defines ERES that are critical for protein ER export, Sec16 …

… of secretory cargo and relevant machinery into budding vesicles at specialised sites on the
endoplasmic reticulum … Here, we define the precise subcellular localisation of mammalian …

… then normalized again by defining the average signal from … the mammalian Sec16
homologues are required for normal … Insight may come from examining the mammalian secretory …

… the initial step in protein secretion. COPII-coated vesicles mediate the export of newly synthesized
… Biochemical studies defining specific interactions between Sec16 and COPII subunits …

… transitional endoplasmic reticulum (tER) or the ER exit sites (… defined in Drosophila Sec16
and responsible for localizing … Ufe-1 export even at the permissive temperature when normal …

… at endoplasmic reticulum exit sites (ERES) to mediate ER-to-Golgi transport. Sec16 is an
essential factor … for Sec16 function to facilitate ER exit of secretory proteins. Total cell extracts of …

… Mammalian endoplasmic reticulum (ER) exit sites export a variety of cargo molecules …
cooperation with Sec16, is required for maintaining functional ER exit sites characterized by …

… coat proteins have largely been defined in yeast. Formation … It has been proposed that Sec16
may act as a scaffold for the … ER exit sites, but others, such as pancreatic secretory proteins, …

… of proteins and lipids from the endoplasmic reticulum (ER), is … We use the term ER exit site
(ERES) to define the structures … membrane protein Sec16 in the organization of ER exit sites. …