… gene transfer in murine cells to human systems. Here we present a method for the transduction
of hES cells with ecotropic retroviral vectors. hES cells were transiently transfected with a …

… stem cell targeted gene transfer for the treatment of human … of sequential transduction with
rAAV and an ecotropic retroviral vector of … and retroviral vectors. One advantage of adenoviral …

… supernatant is then used to achieve high‐level transduction of the ecotropic packaging cell
line GP+… Incubate a further 48–72 h at 37 to allow retroviral vector to transduce NIH‐3T3 cells. …

… competent vectors (RCVs), and transduced T-cell preparations … testing, we transduced
human T cells with ecotropic RVs so … of human hematopoietic progenitor cells with a hybrid EBV/…

… the pluripotent cells of each species. We have examined the properties of the HSF6 and
HES3 human embryonic stem cell lines after transduction with a variety of viral vectors and have …

… a method for transduction of human cells with ecotropic lentivirus, … ecotropic lentivirus with
sufficiently high titer to transduce greater … oncogenes and stem cell biology including iPS cells. …

… Genetic modification of HSPCs using retrovirus vectors … for γ-retroviral vectors are
ecotropic-MLV envelope (Eco, … with embryonic stem cells, but is also observed in other cell types. …

… generation of retroviral vectors can readily transduce a significant proportion of mouse stem
cells,1… with murine ecotropic retroviral vectors leading to sustained transgene expression. …

… Despite their inability to transduce non-cycling cells, retroviral vectors derived from MLV …
(LTRs; dotted arrows) of murine embryonic stem cell virus (MSCV) and the packaging signal Ψ …

… For the packaging of the retroviruses we used the ecotropic TE-FLY-E packaging cell line,
the … with results recently reported for transduction of fluorescent markers into ES cells using …