… show that the effects of chloride or carbon dioxide on decreasing the affinity of hemoglobin
for … Our results have implications for the allosteric mechanism of oxygen regulation. Were the …

… role of tertiary and quaternary allosteric effects may be better … R Hb and are locked by ionic
interactions (salt bridges) in … variation is the absence of a water molecule in the heme pocket …

… The allosteric effectors, 2,3-BPG and IHP, significantly affect … behind an Fe 3+ ion for reacting
with water to form aquomet-… the Fe 2+ ion of the deoxy-Mb to interact with water. The aquo-…

… or exogenous ligands or effectors affect Hb allostery. … Hb \( \zeta_{2} \beta_{2}^{s} \) inhibits
polymerization of deoxygenated … chloride ions are found in the larger T structure central water …

… at a high ionic strength of 2 M phosphate (s), (9) and (4) ionic … described above, polymerization
mutation effects are defined … significant mutation effects on hemoglobin oxygen binding …

… hemoglobin, an allosteric protein in which reduced water … chromatography to strip small ions
and the organic phosphate, 2,3… sodium phosphate buffer stock gave polymer solutions of 10, …

… hemoglobin is the rate-limiting step of Hb cooperative reaction. This evidences that water
binding is a crucial step on the allosteric … After the original studies of water effects on allosteric …

… effect of solvated O 2 supports our earlier observation for the allosteric regulation of hemoglobin…
that such ions are essential and water molecules around the ions may be unimportant for …

… dioxide, chloride ions also contribute to the Bohr effect by … to bind deeper in the central
water cavity in both the T and R … inhibited deoxygenated sickle Hb polymerization, resulting in …

… and function of hemoglobin, and analyzes the effect of point … than the heme, with hydrogen
ions, carbon dioxide, chloride (and … or ions bias the allosteric transition and are thus called the …