3D differential phase-contrast microscopy with computational illumination using an LED array
We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational
illumination with a programmable LED array. By capturing intensity images with various
illumination angles generated by sequentially patterning an LED array source, we digitally
refocus images through various depths via light field processing. The intensity differences
from images taken at complementary illumination angles are then used to generate DPC
images, which are related to the gradient of phase. The proposed method achieves 3D DPC …
illumination with a programmable LED array. By capturing intensity images with various
illumination angles generated by sequentially patterning an LED array source, we digitally
refocus images through various depths via light field processing. The intensity differences
from images taken at complementary illumination angles are then used to generate DPC
images, which are related to the gradient of phase. The proposed method achieves 3D DPC …
We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational illumination with a programmable LED array. By capturing intensity images with various illumination angles generated by sequentially patterning an LED array source, we digitally refocus images through various depths via light field processing. The intensity differences from images taken at complementary illumination angles are then used to generate DPC images, which are related to the gradient of phase. The proposed method achieves 3D DPC with simple, inexpensive optics and no moving parts. We experimentally demonstrate our method by imaging a camel hair sample in 3D.
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