A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues
Analytical Biochemistry, 2006•iris.unito.it
In summary, we have adapted the CTAB method to the extraction of LMW RNA from
polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and
intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for
siRNA detection and miRNA expression analysis by northern blotting, and to produce small
RNA libraries from plant organs, such as fruits and seeds, in which the role of these small
RNA molecules has been, thus far, little explored.
polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and
intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for
siRNA detection and miRNA expression analysis by northern blotting, and to produce small
RNA libraries from plant organs, such as fruits and seeds, in which the role of these small
RNA molecules has been, thus far, little explored.
In summary, we have adapted the CTAB method to the extraction of LMW RNA from polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for siRNA detection and miRNA expression analysis by northern blotting, and to produce small RNA libraries from plant organs, such as fruits and seeds, in which the role of these small RNA molecules has been, thus far, little explored.
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