A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile …
Y Zhao, P Chen, X Liao, B Zhou, J Liao, Z Huang… - Molecular …, 2013 - Springer
Abstract mtDNA was isolated from cytoplasmic male sterility (CMS) line P3A and its
maintainer P3B of kenaf (Hibiscus cannabinus L.). The atp9 gene and its two flanking
sequences were obtained using homology cloning and high-efficiency thermal asymmetric
interlaced PCR methods. The coding sequences showed only two base pairs difference
between the CMS and its maintainer, and shared a homology of over 87% with atp 9 genes
from other species in GenBank. However, when comparing the flanking sequences, a 47-bp …
maintainer P3B of kenaf (Hibiscus cannabinus L.). The atp9 gene and its two flanking
sequences were obtained using homology cloning and high-efficiency thermal asymmetric
interlaced PCR methods. The coding sequences showed only two base pairs difference
between the CMS and its maintainer, and shared a homology of over 87% with atp 9 genes
from other species in GenBank. However, when comparing the flanking sequences, a 47-bp …
A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile …
ZYH Zhao YanHong, CP Chen Peng… - 2013 - cabidigitallibrary.org
MtDNA was isolated from cytoplasmic male sterility (CMS) line P3A and its maintainer P3B
of kenaf (Hibiscus cannabinus L.). The atp9 gene and its two flanking sequences were
obtained using homology cloning and high-efficiency thermal asymmetric interlaced PCR
methods. The coding sequences showed only two base pairs difference between the CMS
and its maintainer, and shared a homology of over 87% with atp9 genes from other species
in GenBank. However, when comparing the flanking sequences, a 47-bp deletion was …
of kenaf (Hibiscus cannabinus L.). The atp9 gene and its two flanking sequences were
obtained using homology cloning and high-efficiency thermal asymmetric interlaced PCR
methods. The coding sequences showed only two base pairs difference between the CMS
and its maintainer, and shared a homology of over 87% with atp9 genes from other species
in GenBank. However, when comparing the flanking sequences, a 47-bp deletion was …
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