A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle
J Gomes, M Santos, A Amaro, IP da Fonseca… - Molecular and cellular …, 2017 - Elsevier
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of
Theileria annulata infection in cattle. The results were compared with a real-time PCR used
for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from
16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being
detected in 66% and 67% of the samples, respectively. The results showed that the LAMP
assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of …
Theileria annulata infection in cattle. The results were compared with a real-time PCR used
for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from
16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being
detected in 66% and 67% of the samples, respectively. The results showed that the LAMP
assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of …
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