A genetically encoded cyclobutene probe for labelling of live cells
K Liu, B Enns, B Evans, N Wang, X Shang… - Chemical …, 2017 - pubs.rsc.org
We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-
specific incorporation of a cyclobutene-containing amino acid into proteins in response to an
amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins
in live cells was demonstrated using the inverse electron demand Diels–Alder reaction with
a tetrazine.
specific incorporation of a cyclobutene-containing amino acid into proteins in response to an
amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins
in live cells was demonstrated using the inverse electron demand Diels–Alder reaction with
a tetrazine.
[引用][C] A genetically encoded cyclobutene probe for labelling of live cells
B Enns, B Evans, N Wang, X Shang, W Sittiwong… - 2017
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