In this work, Polycaprolactone (PCL), silver nitrate (AgNO3) and zinc oxide (ZnO) were used for fabrication of a multilayered antibacterial nanocomposite material using co-axial electrospinning (CAE). 5, 10, and 15 wt.% concentrations of PCL were utilized, and varying amounts of AgNO3/ZnO were used in all samples to increase antibacterial activity. Products were analysed using Scanning Electron Microscopy (SEM) to obtain the morphological characterization, and Fourier Transform Infrared Spectroscopy (FTIR) was used to determine the functional groups of the PCL, AgNO3, and ZnO materials in the electrospun nanocomposites. Tensile strength was also determined for each sample. Additionally, viscosity, density, and electrical conductivity were measured for each prepared solution before the CAE process. The antibacterial activity of the fabricated electrospun nanocomposite materials was tested against Staphylococcus aureus and Eschericha coli strains. A simple nanoscale assembly approach was provided using CAE processing to incorporate a variety of AgNO3 and ZnO functionalities into the nanofibers to fabricate biohybrid materials for antibacterial surface applications.