A protease isolated from the latex of Plumeria rubra Linn (Apocynaceae) 1: purification and characterization
Methods: Protease was isolated from the latex of Plumeria rubra Linn using acetone
precipitation method and purified by a sequence of DEAE cellulose column
chromatography, followed by two successive column purification in Sephadex G-50 and
Sephadex G-200. The molecular weight of the purified protease was determined by sodium
dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE). The protease was given a
trivial name, Plumerin-R. Results: Plumerin-R showed a single protein band on SDS-PAGE …
precipitation method and purified by a sequence of DEAE cellulose column
chromatography, followed by two successive column purification in Sephadex G-50 and
Sephadex G-200. The molecular weight of the purified protease was determined by sodium
dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE). The protease was given a
trivial name, Plumerin-R. Results: Plumerin-R showed a single protein band on SDS-PAGE …
A protease isolated from the latex of Plumeria rubra Linn (Apocynaceae) 1: purification and characterization.
IC Indranil Chanda, SK Basu, SK Dutta, SRC Das - 2011 - cabidigitallibrary.org
Purpose: To isolate, purify and characterize protease from the latex of the plant. Methods:
Protease was isolated from the latex of Plumeria rubra Linn using acetone precipitation
method and purified by a sequence of DEAE cellulose column chromatography, followed by
two successive column purification in Sephadex G-50 and Sephadex G-200. The molecular
weight of the purified protease was determined by sodium dodecyl sulfate polyacrylamide
gel electrophoresis (SDS-PAGE). The protease was given a trivial name, Plumerin-R …
Protease was isolated from the latex of Plumeria rubra Linn using acetone precipitation
method and purified by a sequence of DEAE cellulose column chromatography, followed by
two successive column purification in Sephadex G-50 and Sephadex G-200. The molecular
weight of the purified protease was determined by sodium dodecyl sulfate polyacrylamide
gel electrophoresis (SDS-PAGE). The protease was given a trivial name, Plumerin-R …
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