A set of BEST triple-resonance experiments for time-optimized protein resonance assignment
A series of sequential, intra-residue, and bi-directional BEST H–N–CA, H–N–CO, and H–N–
CB pulse sequences is presented that extends the BEST concept introduced recently for fast
multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the
complete set of experiments required for sequential resonance assignment. We demonstrate
for the protein ubiquitin that 3D BEST H–N–C correlation spectra can be recorded on a
600MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of …
CB pulse sequences is presented that extends the BEST concept introduced recently for fast
multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the
complete set of experiments required for sequential resonance assignment. We demonstrate
for the protein ubiquitin that 3D BEST H–N–C correlation spectra can be recorded on a
600MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of …
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