[PDF][PDF] Acute toxicity studies of the lyophilized aqueous extract of Psychotria microphylla leaf on Clarias gariepinus juveniles

OU Orji, UA Ibiam, PM Aja - International Journal of Biology and …, 2014 - researchgate.net
International Journal of Biology and Biological Sciences, 2014researchgate.net
(LC50) at 96 h of exposure. Six graded concentrations of 0, 0.27, 0.33, 0.47, 0.67 and 1.33
mg/l of the lyophilized extract were applied to C. gariepinus juveniles (mean weight: 180 g
and length: 25 cm) in plastic containers. The 24, 48, 72 and 96 h LC50 values (with 95%
confidence limits) estimated by probit analysis were 0.65 (0.57-0.77), 0.53 (0.45-0.65), 0.41
(0.33-0.49) and 0.35 (0.26-0.42) mgl-1, respectively. During exposure, fish exhibited
discolouration, gulping for air, erratic swimming, loss of reflexes, slow opercular movement …
(LC50) at 96 h of exposure. Six graded concentrations of 0, 0.27, 0.33, 0.47, 0.67 and 1.33 mg/l of the lyophilized extract were applied to C. gariepinus juveniles (mean weight: 180 g and length: 25 cm) in plastic containers. The 24, 48, 72 and 96 h LC50 values (with 95% confidence limits) estimated by probit analysis were 0.65 (0.57-0.77), 0.53 (0.45-0.65), 0.41 (0.33-0.49) and 0.35 (0.26-0.42) mgl-1, respectively. During exposure, fish exhibited discolouration, gulping for air, erratic swimming, loss of reflexes, slow opercular movement and ultimately settling at the bottom motionless just before death. The toxicity of the aqueous extract of the plant against C. gariepinus was both time and dose dependent. It is thought that this plant extract or powder will be useful in aquaculture to eradicate predators and competing wild fish from nursery, rearing and stocking ponds prior to the stocking of commercially grown fry and fingerlings of desired species.
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