Biological molecules are often highly dynamic, and this flexibility can be critical for function.
The large range of sampled timescales and the fact that many of the conformers that are
continually explored are only transiently formed and sparsely populated challenge current
biophysical approaches. Solution nuclear magnetic resonance (NMR) spectroscopy has
emerged as a powerful method for characterizing biomolecular dynamics in detail, even in
cases where excursions involve short-lived states. Here, we briefly review a number of NMR …