The purpose of this study is to examine the instructional high school chemistry videos on EBA in line with Gagne’s nine events of instruction. Content analysis was used to examine the chemistry videos on EBA are appropriate in accordance with Gagne’s nine events of instruction. Content analysis can be conducted in both quantitative and qualitative ways. Hence, a quantitative media content analysis was adopted as a method of design considering the aim of study. Four instructional designers, who are experts in the field, evaluated the appropriateness of the instructional videos. Two different equal groups were formed. Both of them independently evaluated the qualities of the videos according to the phases of Gagne’s Nine Events of Instruction. A scoring rubric was developed by the researchers in order to evaluate the quality of each video on the basis of those phases. Each learning phase in the instructional videos were checked and scored by the experts. Findings indicated that there was a certain need to improve the quality of chemistry videos on EBA website. Consequently, the instructional videos on EBA found not good enough to promote a positive attitude towards the chemistry class, because some phases of the nine events of instruction are missing or needs to be improved.