Primary hepatocytes are used extensively in liver in vitro research, especially in glucose
metabolism studies. A base technique has been adapted based on different needs, like time,
labor, cost, and primary hepatocyte usage, resulting in various primary hepatocyte isolation
protocols. However, the numerous steps and time-consuming reagent preparations in
primary hepatocyte isolation are major drawbacks for efficiency. After comparing different
protocols for their pros and cons, the advantages of each were combined, and a rapid and …