This study reports for the first time a preliminary phytochemical and biological screening of ethanolic extract (EE) from roots of Eremurus persicus (Jaub and Spach) Boiss, a Kurdish herbal remedy for treating inflammatory disorders. The phytochemical analysis was performed by thin layer chromatography and it revealed the presence of flavonoids and antracenones. The total phenolic compounds were also determined. Free radical scavenging (FRS) effect was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and the anti-inflammatory activity investigated by evaluating the inhibition of in vitro lymphocyte proliferation. To exclude citotoxic effect of EE, cell viability tests were also performed. Overall, biological investigation showed that the E. persicus (Jaub and Spach) Boiss root EE possess an interesting anti-inflammatory activity without cytotoxic effects.