The paper deals with the significance of these roots in traditional medical systems with respect to their antimicrobial and photochemical. The qualitative analysis showed the presence of various phytochemicals like flavonoids, alkaloids, phenols, saponins, tannins. Methanol extracts of the dried root Hemidesmus indicus (L.) R. Br.(Indian sarsaparilla) were analysed for their physiochemical constituents. Further study some of these phytochemicals were also quantitatively estimated. The antibacterial properties of Methonal Extracts root material on agar plates was investigated in the present study. The antibacterial activity of Plant Extract was evaluated against on Gram Positive and negative bacteria strains.