[HTML][HTML] Antibiotic resistance pattern of Pseudomonas spp. from patients in a tertiary hospital in South-West Nigeria
Germs, 2021•ncbi.nlm.nih.gov
Methods One hundred and fifty Pseudomonas spp. from different clinical specimens were
obtained from the Obafemi Awolowo University Teaching Hospitals Complex Ile-Ife. Culture
was carried out on MacConkey and blood agar while phenotypic characterization was done
by Gram staining, oxidase, and catalase test. Species identification was done using
MICROBACT TM 24E bacterial identification kit and confirmed by 16S rDNA polymerase
chain reaction (PCR) assay. Antibiotic susceptibility testing to eight antibiotics in four classes …
obtained from the Obafemi Awolowo University Teaching Hospitals Complex Ile-Ife. Culture
was carried out on MacConkey and blood agar while phenotypic characterization was done
by Gram staining, oxidase, and catalase test. Species identification was done using
MICROBACT TM 24E bacterial identification kit and confirmed by 16S rDNA polymerase
chain reaction (PCR) assay. Antibiotic susceptibility testing to eight antibiotics in four classes …
Methods
One hundred and fifty Pseudomonas spp. from different clinical specimens were obtained from the Obafemi Awolowo University Teaching Hospitals Complex Ile-Ife. Culture was carried out on MacConkey and blood agar while phenotypic characterization was done by Gram staining, oxidase, and catalase test. Species identification was done using MICROBACT TM 24E bacterial identification kit and confirmed by 16S rDNA polymerase chain reaction (PCR) assay. Antibiotic susceptibility testing to eight antibiotics in four classes was done.
Results
Pseudomonas aeruginosa was the most frequently occurring species (96.0%); P. putida (2.67%) and P. fluorescens (0.67%) were also identified as well as an isolate of Burkholderia pseudomallei (0.67%). The highest resistance rate among isolates was observed towards gentamicin (35.4%); piperacillin/tazobactam was the most active antibiotic. Multidrug-resistant (MDR) strains constituted 12.8% of the isolates and most MDR strains also displayed a high multiple antibiotic resistance index (MAR).
Conclusions
Pseudomonas aeruginosa is emerging as a highly MDR pathogen in our hospital setting. This calls for the establishment of a surveillance system and antimicrobial stewardship programme in place. Furthermore, we propose a review of the current antibiotics prescription policy, and infection control programmes (ICPs) if we must control the spread of MDR-P. aeruginosa in this environment.
ncbi.nlm.nih.gov
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