Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 1. Assessing analytical validation

MC Ruebelt, NK Leimgruber, M Lipp… - Journal of agricultural …, 2006 - ACS Publications
… rapidly developing, proteome analysis based on two-dimensional gel electrophoresis (2DE) …
and quantitative analysis of the seed proteome of Arabidopsis thaliana with the following …

Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 2. Assessing natural variability

MC Ruebelt, M Lipp, TL Reynolds… - Journal of agricultural …, 2006 - ACS Publications
… in codon changes. Such heritable changes in the DNA directly affect the proteome unless …
or glycosylation sites, and (4) alteration of the degradation stability of the resulting protein. …

Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of gentically modified crops. 3. Assessing unintended effects

MC Ruebelt, M Lipp, TL Reynolds… - Journal of Agricultural …, 2006 - ACS Publications
… An optimized two-dimensional gel electrophoresis (2DE) method was used as a … of
proteomics technology to identify unintended or intended changes in the seed proteome due to …

Twodimensional gel electrophoresis and mass spectrometry for biomarker discovery

D Penque - Proteomics–Clinical Applications, 2009 - Wiley Online Library
… 2-DE gels. ICAT/2-DE/MS was applied for example to quantify proteomic alterations induced
by … NBS/2-DE/MS was used to profile proteomic alterations in breast cancer cells induced by …

Comparative skeletal muscle proteomics using two-dimensional gel electrophoresis

S Murphy, P Dowling, K Ohlendieck - Proteomes, 2016 - mdpi.com
… Below sections review the application of the 2D-IEF/SDS-PAGE method in modern
proteomics and its modifications for comparative studies using fluorescent dyes. Included are …

The evolution of two-dimensional gel electrophoresis-from proteomics to emerging alternative applications

PY Lee, N Saraygord-Afshari, TY Low - Journal of Chromatography A, 2020 - Elsevier
modifications to the original 2-DE method that open new door of opportunities for new useful
applications… 2D BN/SDS-PAGE has been exploited to interrogate different aspects of protein …

Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis: novel protein markers in hepatocellular carcinoma tissues

W Sun, B Xing, Y Sun, X Du, M Lu, C Hao, Z Lu… - … & cellular proteomics, 2007 - ASBMB
… In summary, we profiled proteome alterations in HCC tissues, … tissue by two-dimensional gel
electrophoresis and matrix … /mass spectra were used to interrogate human sequences in the …

Proteomic expression analysis of colorectal cancer by twodimensional differential gel electrophoresis

P Alfonso, A Núñez, J Madoz‐Gurpide… - …, 2005 - Wiley Online Library
… MS coupled with database interrogation as being differentially … The use of the mixed internal
standard in this experimental … leads to marked cytoskeletal changes and to alterations in the …

Use of twodimensional gel electrophoresis in predictive toxicology: Identification of potential early protein biomarkers in chemically induced hepatocarcinogenesis

K Fella, M Glückmann, J Hellmann, M Karas… - …, 2005 - Wiley Online Library
… Furthermore, we raise the question: can the overlapping … changes on the protein level,
occurring at different timepoints in NNM exposed rat liver, can be detected by a proteomic

Application of proteomics in cancer gene profiling: two-dimensional difference in gel electrophoresis (2D-DIGE)

D Hariharan, ME Weeks… - Cancer Gene Profiling …, 2010 - Springer
… , proteomics has been increasingly used in cancer research ( 1 ). Proteomics encompasses
identification of changes in … of proteomic techniques are available to interrogate the cancer …