Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 1. Assessing analytical validation
MC Ruebelt, NK Leimgruber, M Lipp… - Journal of agricultural …, 2006 - ACS Publications
… rapidly developing, proteome analysis based on two-dimensional gel electrophoresis (2DE) …
and quantitative analysis of the seed proteome of Arabidopsis thaliana with the following …
and quantitative analysis of the seed proteome of Arabidopsis thaliana with the following …
Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 2. Assessing natural variability
MC Ruebelt, M Lipp, TL Reynolds… - Journal of agricultural …, 2006 - ACS Publications
… in codon changes. Such heritable changes in the DNA directly affect the proteome unless …
or glycosylation sites, and (4) alteration of the degradation stability of the resulting protein. …
or glycosylation sites, and (4) alteration of the degradation stability of the resulting protein. …
Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of gentically modified crops. 3. Assessing unintended effects
MC Ruebelt, M Lipp, TL Reynolds… - Journal of Agricultural …, 2006 - ACS Publications
… An optimized two-dimensional gel electrophoresis (2DE) method was used as a … of
proteomics technology to identify unintended or intended changes in the seed proteome due to …
proteomics technology to identify unintended or intended changes in the seed proteome due to …
Two‐dimensional gel electrophoresis and mass spectrometry for biomarker discovery
D Penque - Proteomics–Clinical Applications, 2009 - Wiley Online Library
… 2-DE gels. ICAT/2-DE/MS was applied for example to quantify proteomic alterations induced
by … NBS/2-DE/MS was used to profile proteomic alterations in breast cancer cells induced by …
by … NBS/2-DE/MS was used to profile proteomic alterations in breast cancer cells induced by …
Comparative skeletal muscle proteomics using two-dimensional gel electrophoresis
S Murphy, P Dowling, K Ohlendieck - Proteomes, 2016 - mdpi.com
… Below sections review the application of the 2D-IEF/SDS-PAGE method in modern
proteomics and its modifications for comparative studies using fluorescent dyes. Included are …
proteomics and its modifications for comparative studies using fluorescent dyes. Included are …
The evolution of two-dimensional gel electrophoresis-from proteomics to emerging alternative applications
… modifications to the original 2-DE method that open new door of opportunities for new useful
applications… 2D BN/SDS-PAGE has been exploited to interrogate different aspects of protein …
applications… 2D BN/SDS-PAGE has been exploited to interrogate different aspects of protein …
Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis: novel protein markers in hepatocellular carcinoma tissues
W Sun, B Xing, Y Sun, X Du, M Lu, C Hao, Z Lu… - … & cellular proteomics, 2007 - ASBMB
… In summary, we profiled proteome alterations in HCC tissues, … tissue by two-dimensional gel
electrophoresis and matrix … /mass spectra were used to interrogate human sequences in the …
electrophoresis and matrix … /mass spectra were used to interrogate human sequences in the …
Proteomic expression analysis of colorectal cancer by two‐dimensional differential gel electrophoresis
P Alfonso, A Núñez, J Madoz‐Gurpide… - …, 2005 - Wiley Online Library
… MS coupled with database interrogation as being differentially … The use of the mixed internal
standard in this experimental … leads to marked cytoskeletal changes and to alterations in the …
standard in this experimental … leads to marked cytoskeletal changes and to alterations in the …
Use of two‐dimensional gel electrophoresis in predictive toxicology: Identification of potential early protein biomarkers in chemically induced hepatocarcinogenesis
K Fella, M Glückmann, J Hellmann, M Karas… - …, 2005 - Wiley Online Library
… Furthermore, we raise the question: can the overlapping … changes on the protein level,
occurring at different timepoints in NNM exposed rat liver, can be detected by a proteomic …
occurring at different timepoints in NNM exposed rat liver, can be detected by a proteomic …
Application of proteomics in cancer gene profiling: two-dimensional difference in gel electrophoresis (2D-DIGE)
D Hariharan, ME Weeks… - Cancer Gene Profiling …, 2010 - Springer
… , proteomics has been increasingly used in cancer research ( 1 ). Proteomics encompasses
identification of changes in … of proteomic techniques are available to interrogate the cancer …
identification of changes in … of proteomic techniques are available to interrogate the cancer …