Gold (Au) nanoribbons were synthesized in aqueous phase under ambient conditions by using dimethylene bis(tetradecyldimethyl-ammonium bromide) (14-2-14) as a capping agent as well as a soft template. A two steps seed-growth (S-G) method was used. The first step of S-G method mainly gave nanorods of high aspect ratio along with nanoparticles of other shapes, but the next step produced mainly fine Au nanoribbons of several micrometers long, 50–150 nm wide, and ≈5 nm thick. They were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, energy dispersive X-ray, and X-ray diffraction analysis. Isotropic liquid crystalline phase of 14-2-14 provided a soft template effect and derived nanorods of high aspect ratios toward nanoribbon formation at room temperature.