Human peripheral blood mononuclear cells were isolated and assessed for the presence of
contaminating polymorphonuclear leukocytes and platelets. Incubations of these cell
isolates were performed in the presence or absence of the calcium ionophore A23187
and/or 1-14C-labeled or unlabeled arachidonic acid. Using reverse phase high pressure
liquid chromatography with simultaneous monitoring of ultraviolet light absorption at 229
and 280 nm and, where appropriate, of radioactivity, our studies reveal that human …

Human peripheral blood mononuclear cells were isolated and assessed for the presence of
contaminating polymorphonuclear leukocytes and platelets. Incubations of these cell
isolates were performed in the presence or absence of the calcium ionophore A23 187
and/or l-14C-labeled or unlabeled arachidonic acid. Using reverse phase high pressure
liquid chromatography with simultaneous monitoring of ultraviolet light absorption at 229
and 280 nm and, where appropriate, of radioactivity, our studies reveal that human …