Sequential earthquakes have severe destruction on structures, including the accumulative structural and nonstructural damage, compared to single earthquakes and due to the lack of sufficient opportunity to repair of the structure, the possibility of structural damage increases. In this research, the effect of seismic sequence on the relatively new system of reinforced concrete frames equipped with steel plate shear walls has been investigated. Based on this, four systems of 4, 8, 12 and 24 stories, which represent short, intermediate, tall, are modeled in finite element software and subject to four sets of the single and sequential earthquakes and with a variety of application methods. Sequential earthquakes, including real, repetitive and randomized methods, are subjected to non-linear dynamic analysis under four sets of single and sequential acceleration. The seismic scenarios used include sequential recorded critical earthquakes. The analysis showed that the predominant period of the aftershock significantly influences the post main shock response. Real seismic sequence increases the ratio of peak inter-story drift by an average of 2 times the similar demand in a single earthquake and increases the ratio of maximum ductility demand by 1.52 times in the structure. In artificial sequence, the ratio of peak maximum inter-story drift demand increase is in 100%, 150% and 200% aftershocks. In the iteration method, it is equal to 1.2, 2.0 and 2.6 times the single earthquake. Aftershocks may change the direction and magnitude of residual displacement in real and artificial seismic sequences. Continuation of the equation to calculate the demand for seismic sequence ductility was extracted.