This cross sectional study was conducted at the maternity unit of the University of Uyo Teaching Hospital, Uyo. The aim was to determine the degree of awareness and practice of malaria prevention strategies among antenatal attendees in Uyo, South-South Nigeria. Semi-structured questionnaires were administered to two hundred and fifty pregnant women in the antenatal clinic. Majority of the respondents were 21-30 years (67.6%), married (98.8%) and 90.0% had at least secondary level education. About 71.2% of the respondents knew that malaria had adverse effects in pregnancy (p<. 001) while 76.0% of them had received treatment for malaria during pregnancy (p<. 001). 76.4% of the respondents had heard of Insecticide Treated Bed Nets (ITNs)(p<. 001) while only 16.4% had used them (p<. 001). Only 40.8% of the patients had heard of Intermittent Preventive Treatment (IPT)(p<. 001) while 12.0% received drugs under IPT. The level of utilization of ITNs and IPT in our environment is low. Involvement of the mass media in community enlightenment programs, ensuring the distribution of ITNs and drugs for IPT at no cost or highly subsidized rates in all antenatal clinic outlets, and organisation of regular updates on currents trends in malaria preventive measures for all health staff involved in maternity care are advocated.