The identification of axonal mRNAs in model organisms has led to the discovery of multiple
proteins synthesized within axons for functional roles such as axon guidance and injury
response. The extent to which protein synthesis within the axon is conserved in humans is
unknown. Here we used axon-isolating microfluidic chambers to characterize the axonal
transcriptome of human embryonic stem cells (hESC-neurons) differentiated using a
protocol for glutamatergic neurons. Using gene expression analysis, we identified mRNAs …