… by delivering a catalytically active polypeptide fragment of the … the intracellular proteolytic
step that produces the 37-kDa … with a furin cDNA regained sensitivity to PE and to viruses (54). …

… the recombinant protein within the bacterial host. For example, … However, when cells are
producing the protease-sensitive … secretion of three chimeric polypeptides in Escherichia coli. J …

… Apart from the EspA polypeptides of 22 and 25 kDa, two … bacterial supernatants was never
detected in extracts of infected cells, which may indicate that EspA is processed proteolytically …

… of the polypeptide chains, … bacteria are cultured at a low temperature (ie, 16 to 18C) (6, 14),
we demonstrated that the E. coli cpn + strain was more efficient (in terms of rate of production …

… release of the resulting polypeptides, which we hypothesize is … was proteolytically removed
from a majority of the ACT … examined the ability of bacterial strains producing ACT with aa …

… Bacteria are the most efficient producers, but they are not able … After translocation into the
ER, nascent polypeptides are … recombinant hGH, a proteolytically sensitive model protein, …

… strains deficient in DegP have frequently been constructed, and this resulted in improved
yield of proteolytically sensitive … regulate functional properties of bacterial strains. Although we …

… of a bacterial promoter, and express the protein in a bacterial host. Most proteins can be
produced in … High-level expression of a proteolytically sensitive diphtheria toxin fragment in …

… When producing mammalian proteins in a bacterial host such … refolding of When a
proteolytically sensitive site is located in a … degradation and secretion of three chimeric …

… producing cell and is bioactive, inhibiting sensitive cells in the same way as E. coli microcin
C. Proteolytic processing inside producing … is split into two separate polypeptides encoded by …