Bio-fabrication of ZnO-CuO nanoporous composite was successfully synthesized using Gum Arabic. The morphology, elemental composition, surface plasmon resonance and functional groups bonds of the ZnO-CuO nanoporous composite were confirmed by SEM, EDX, UV-Visible and FTIR techniques respectively. Nanolarvicidal activity of ZnO-CuO nanoporous composite was tested against 1st, 2nd, 3rd, and 4th instars of malaria vectors for 24 hours using different concentrations. The lethal concentrations (LC50) of ZnO-CuO nanoporous composite against 1st, 2nd, 3rd, and 4th instars were found to be 8.841, 8.734, 8.963 and 10.557 mg/L respectively. Whereas, the LC90 of ZnO-CuO nanoporous composite against 1st, 2nd, 3rd, and 4th instars were found to be 19.062, 28.063, 40.888 and 79.567 mg/L respectively. For all instars, the correlation coefficients were found