The objective of this study is to develop a method of sustainability assessment for ranking the prior order of biomass-based conversion technologies for the production of biofuel. Both tactical and environmental issues make biomass a vital energy source and part of sustainable energy policy. A multi-criteria decision-making process permits the decision makers to use multiple variables to assess its consequences on biofuel production. In this study, five criteria relevant to environmental and technological aspects have been taken for sustainability assessment. This proposed method considered two biomass-based technologies such as conventional pyrolysis and gasification, and biomass pyrolysis has been taken as the most sustainable process, since it gives three types of biofuels (bio-oil, char and biogas) and can be chosen for further development. This study suggests a multi-criteria decision-making framework for prioritization of biomass materials based on multi-objective optimization on the basis of ratio analysis (MOORA) method. During this evaluation, the performance of the reference point approach and full multiplicative MOORA method are also tested for this problem. The study concluded that all these applied methods are very simple to understand, and easy to implement and endow with almost exact rankings.