Biosynthesis of (−)-ent-kaurenoic acid in Smallanthus sonchifolius and its effect against microbial biofilms

AA Lopes, ES Pina, TT Nader, FB Da Costa… - Phytochemistry …, 2016 - Elsevier
Phytochemistry Letters, 2016Elsevier
Abstract The biosynthetic pathway of (–)-ent-kaurenoic acid (1) was investigated by
incorporation of 1-d-13 C-glucose in Smallanthus sonchifolius (Asteraceae) plantlets. The
13 C-enrichment pattern indicated that methylerythritol-4-phosphate (MEP) pathway is the
biosynthetic pathway involved in diterpenoid biosynthesis. Our studies in S. sonchifolius
reinforce that the biosynthesis of different classes of terpenes should not be
compartmentalized into cytosol and plastids. Additionally,(–)-ent-kaurenoic acid showed …
Abstract
The biosynthetic pathway of (–)-ent-kaurenoic acid (1) was investigated by incorporation of 1-d-13C-glucose in Smallanthus sonchifolius (Asteraceae) plantlets. The 13C-enrichment pattern indicated that methylerythritol-4-phosphate (MEP) pathway is the biosynthetic pathway involved in diterpenoid biosynthesis. Our studies in S. sonchifolius reinforce that the biosynthesis of different classes of terpenes should not be compartmentalized into cytosol and plastids. Additionally, (–)-ent-kaurenoic acid showed antimicrobial activity against Staphylococcus aureus biofilm.
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