… mutated in tumours, raising the possibility that the mutations do not result in a simple loss
of function. Here we show that cancer-associated IDH1 mutations … (-)-2-hydroxyglutarate (2HG). …

… However, the effect of the cancer-associated IDH1 mutations on reductive glutamine metabolism
has not been fully explored. Alterations in the steady-state levels of metabolites derived …

… ) R140Q provide cells with millimolar r-2-hydroxyglutarate (r-2HG) concentrations, whereas
… form submillimolar s-2-hydroxyglutarate (s-2HG). However, even wild-type IDH1 and IDH2, …

… IDH1 mutations cause a gain-of-function, resulting in the production and accumulation of
2-hydroxyglutarate (… Genotyping of 145 AML biopsies identified 11 IDH1 R132 mutant samples. …

… and energy production through generation of NADH. Cancer-associated mutations in
IDH1 and … Mutant enzymes produce (R)-2-hydroxyglutarate, which in turn inhibits αKG-dependent …

… It has been recently reported that cancer-associated IDH1 R132 mutants display a loss-of-function
for the use of isocitrate as substrate, with a concomitant gain-of-function for the …

… different mutants of IDH1 (R132H, R132C, and R132G) [53] show that the IDH1 mutants
indeed have a differential production of (R)-2-HG, similar to our results with the IDH2 mutants. …

… However, on the basis of this study there are at least five reproducible cancer-associated
mutations that can result in 2HG production and these appear to be selected for in several well-…

… Cancer-associated metabolite 2-hydroxyglutarate accumulates in acute myelogenous
leukemia with isocitrate dehydrogenase 1 and 2 mutations. J Exp Med 207: 339–344. …

… IDH1 mutation has a metabolic effect in these cells. We hypothesized that the altered features
in IDH1 mutant … might be increased in the IDH1 mutant dataset from the current experiment. …