Carbonic anhydrase subunits form a matrix-exposed domain attached to the membrane arm of mitochondrial complex I in plants
S Sunderhaus, NV Dudkina, L Jänsch… - Journal of Biological …, 2006 - ASBMB
Complex I of Arabidopsis includes five structurally related subunits representing γ-type
carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these
subunits within complex I was investigated. Direct analysis of isolated subcomplexes of
complex I by liquid chromatography linked to tandem mass spectrometry allowed the
assignment of the CA subunits to the membrane arm of complex I. Carbonate extraction
experiments revealed that CA2 is an integral membrane protein that is protected upon …
carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these
subunits within complex I was investigated. Direct analysis of isolated subcomplexes of
complex I by liquid chromatography linked to tandem mass spectrometry allowed the
assignment of the CA subunits to the membrane arm of complex I. Carbonate extraction
experiments revealed that CA2 is an integral membrane protein that is protected upon …
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