The modern development reveals that the world is facing energy crisis as a result of the dwindling sources of fossil fuels. Biofulel is renewable clean bioenergy which can be made from vegetable oils, micro-algal oil and animal fats. The feasibility of Biofuel production from clove oil and jatropha oil feedstock was studied in the present investigation. In addition, the studies were evaluating the physicochemical properties and thermal stability of fuel blend between clove oil and jatropha oil (JO). In this study, JO-clove oil blend with initial JOC5, JOC10, and JOC15 in percentage 5, 10 and 15% on the basis volume, have been used. Density, viscosity, heating value, flash point, and thermogravimetric analysis were tested by international standard method. The result indicates that the presence of clove oil affect in density and heating value slightly increase while viscosity and flash point decrease. Thermal stability in JO was modified due to presence of clove oil that marked by percentage of mass loss increase and onset temperature decrease. The improvement physicochemical properties and thermal stability caused by molecular interaction between eugenol and triglyceride that makes molecule active in motion. The volatility of clove oil has big contribution in thermal stability of JO beside the percentage of unsaturated fatty acid.